Putin, on an apparent sarcastic note, added that if Comey "continues to be persecuted in this connection, we will be ready to provide political asylum to him", according to various news outlets.
"In this case, he (Comey) is not the head of a special service but a human rights activist who defends a certain position", Putin said.
Putin called the move "really strange" and compared Comey to whistleblower Edward Snowden, who has sought asylum in Russian Federation since 2013 after leaking classified documents from the National Security Agency.
Comey testified last week that he orchestrated the leak of accounts of conversations with US President Donald Trump because he thought it might lead to the appointment of a special prosecutor to lead the Russian Federation investigation into alleged meddling in the 2016 Presidential election.
Acknowledging that the U.S. Senate is set to introduce new sanctions on Russian Federation and to prevent the Trump administration from rolling back the sanctions without congressional support, Putin said: "Whatever the case, I believe this is utterly groundless".
"We know that now there is a bill in the US Senate regarding toughening sanctions. Why?"
The Direct Line is an annual show where viewers can send a broad range of political queries to the Russian leader which he addresses over a course of several hours.
Mr Putin began his answer saying he hadn't followed the testimony closely.
On a conciliatory note, Putin added that Russian Federation still hopes for normalization of ties with the U.S.
"I have grandchildren. They live normal lives", Putin said during his annual marathon TV call-in with Russians, confirming a revelation from a series of interviews by USA filmmaker Oliver Stone that have been airing in the United States this week.
The impacts of sanctions against Russian Federation have not been "drastic" compared to the global conjuncture, President Vladimir Putin said on Thursday.
"We have been through two wars together", Putin said.
"I think it's better not to touch anything", he said.
Putin also noted that Moscow hopes that the USA could play a "constructive role" in helping settle the Ukrainian crisis, an offer which we doubt will be favorably met by the Pentagon. They are republished from a number of sources, and are not produced by MintPress News.
Moscow and Washington can cooperate on issues including the non-proliferation of weapons and the Syria crisis, he added.