Uber's closest USA rival in the taxi-hailing business, Lyft has repeatedly met with officials from London's transport regulator over the past year, a sign it may be targeting the city for global expansion.
Earlier today, Fred Jones, UK head of cities for Uber said that it was "just not clear for us what [TfL's] concerns might be" and described how they were only informed of the decision five minutes before it was announced on Friday.
Transport for London (TfL) cited "public safety and security implications" among its reasons for denying Uber's license.
Uber urged customers to sign the petition in a tweet posted at 13:41 on Friday, about two hours after the London agency's decision.
Transport bosses have since come under pressure to fully explain why Uber's licence was not being renewed amid accusations the decision was politically motivated and had rested on trumped up charges.
Uber now has about 40,000 drivers in London, and is used by about 3.5 million customers, but its rise has sparked the most significant backlash to date against a major champion of the "gig economy". "Obviously I am pleased that he has acknowledged the issues that Uber faces in London", Khan said.
Mayor of London Sadiq Khan welcomed an apology from Khosrowshahi on Monday.
At the time, Uber said it was surprised by the letter and that it had a good working relationship with the police.
The taxis licenses themselves - licensing is controlled, limited in number, and therefore worth a lot - have plummeted in worth since Uber first hit Sydney streets:worth $406,000 in October, 2012, the month before Uber entered the market, they are now worth only $200,000. They are also free to operate while an appeal is ongoing.
"This was one of the unusual things around the TfL notice yesterday is they are the ones who do all of the checks and license the drivers".
Even if Uber is officially banned from London, the 30,000-40,0000 drivers operating there could continue to work while these new rulings are fought in the courthouses.
Uber CEO apologies to Londoners on behalf of company.
While the latter is seemingly aimed at TfL's claims that Uber deliberately under-reported crimes, Khosrowshahi was careful to avoid directly addressing the allegations with legal proceedings underway.